神經(jīng)氨酸酶也稱唾液酸酶（EC 3.2.1.18）。神經(jīng)氨酸酶負(fù)責(zé)催化唾液酸與糖蛋白之間糖苷鍵的水解形成游離的N-乙酰神經(jīng)氨酸，而游離的N-乙酰神經(jīng)氨酸在N-乙酰神經(jīng)氨酸醛縮酶的作用下裂解產(chǎn)生丙酮酸。此酶可與N-乙酰神經(jīng)氨酸醛縮酶、乳酸脫氫酶、丙酮酸氧化酶一起檢測(cè)唾液酸的含量。
Appearance:White powder, lyophilized
Source:Microorganism
Enzyme Commission Number: EC 3.2.1.18
CAS Number:9001-67-6
Storage temperature:-20℃
Specific activity:≥ 300U/mg protein
Unit definition: One unit will deaminated one micromole of N-Acetylneuraminy-R to N-Actylneuraminate per minute at pH 7.5 at 37℃.
PROPERTIES
Molecular weight: 52 kDa (SDS-PAGE)
Isoelectric point: 5.86
Michaelis constant: 1.02mM(sialyllactose pH6.5)
Optimum pH: 5.0
Optimum temperature: 50℃
pH Stability: 4.0~10.0 (25℃, 25hr)
Thermal stability: < 40℃ (pH 7.5, 10min)